Assessment of potentiality of bifidobacteria Animalis Subsp Lactis. (HBM-NIN-06) isolated from human breast milk and its use in the development of probiotic curd

Bifido's are widely used in the treatment of many diseases both in humans and animals in addition to their incorporation in aquatic feedsetc, therefore keeping this in view we have made an attempt to isolate this probiotic micro flora of human origin which may have colonization in human intestine and prevent or protect from common diseases viz.diarrhoea, irritable bowel syndrome, constipation, etc., including immunomodulation effects of both mucosal and systemic immunity. The Bifidobacterium animalis sub sps lactis (HBM-NIN-06) was isolated and identified from human breast milk by morphology, Gram's staining and biochemical tests, etc. This was further confirmed for Genus and Species by molecular methods (PCR) using specific primers. This Bifido had good probiotic potentials such as resistance to pH, bile salt tolerance at 0.3%, resistance to number of routinely prescribed antibiotics in addition this has shown to have anti-pathogenic effects onsome food borne pathogens. Based on these observations we have developed a probiotic curd using starter cultures of Lactobacillus bulgaricus sub sps delbruki (UBLB-38) and Streptococcus thermophilus (UBST-50) with incorporation of the above isolated Bifidobacterium animali (HBM-NIN-06). This Probiotic curd was assessed for its sensory evaluation on human volunteers and was found to have 90% acceptability andtheir viability retained upon storage at 4-8 °C for about one month Therefore the Bifidobacterium animalis subsps lactis which is of human origin and from Indian women may have immense health benefits therefore we have developed a food supplement which may be valuable for therapeutical application on human health.