Method for separating and analyzing volatile and semi-volatile flavor components in tobacco by using liquid chromatography-gas chromatography/mass spectrometry technology

A method for separating and analyzing volatile and semi-volatile flavor components in tobacco by using liquid chromatography-gas chromatography/mass spectrometry technology comprises the following steps: 1. taking a given weight of tobacco products and extracting the volatile and semi-volatile flavor components from the tobacco by simultaneously adopting distillation and extraction; 2. concentrating the extracted samples with a concentrator; 3. carrying out group component separation on the concentrated extracted samples by normal-phase liquid chromatography and collecting distillates by different time sections; and 4. concentrating the collected distillates by the concentrator and respectively entering a gas chromatography-mass spectrometer (GC-MS) for detailed analysis. Compared with theprior art, the method of the invention can effectively extract the volatile and semi-volatile flavor components from the tobacco, carry out group component separation on the extract liquor by normal-phase liquid chromatography, then carry out accurate GC-MS qualitative and quantitative analysis on each group component according to different boiling points, not only has good selectivity and high separation efficiency but also ensures more accurate qualitative and quantitative analysis of each component.