Additional file 2: Figure S2. of Assessment of cytotoxicity exerted by leaf extracts from plants of the genus Rhododendron towards epidermal keratinocytes and intestine epithelial cells

Overview of plasma membrane integrity and apoptotic cell death induced by 24h exposure of HaCaT keratinocytes to three different concentrations (5, 50, and 500 μg/mL) of Rhododendron leaf extracts. Single channel fluorescence, phase contrast and merged micrographs taken with a confocal laser scanning microscope. Violet signals in merged pictures are due to overlapping red, PI-derived signals with blue Draq5™ staining of the nuclei. Cells treated with 0.5 % DMSO served as controls, A) R. hippophaeoides var. hippophaeoides, B) R. minus, C) R. rubiginosum, D) R. cinnabarinum, E) R. ferrugineum, F) R. polycladum, G) R. concinnum, H) R. xanthostephanum, I) R. anthopogon ssp. anthopogon, J) R. ambiguum, K) R. hirsutum, and L) R. racemosum. Bar represents 250 μm. (TIFF 6203 kb)