Effect of quercetin loaded liposomes or nanostructured lipid carrier (NLC) on post-thawed sperm quality and fertility of rooster sperm.

Abstract The aim of this study was to investigate the effects of 10, 15 and 20 μM quercetin, alone or loaded in nanoliposomes or in nanostructured lipid carrier (NLC) on sperm rooster cryopreservation and fertility performance. Sperm motility, viability, abnormalities, membrane integrity, mitochondrial activity, apoptosis status, lipid peroxidation (LP), GPX, SOD, TAC and fertility and hatchability rate were investigated after freeze-thawing. A significantly higher percentage (P   0.05) affected by different levels of quercetin. Fertility and hatchability rate showed higher levels in 15 μM quercetin and quercetin loaded NLC group compared with control group. In conclusion, it seems that quercetin loaded NLC enhanced the antioxidant effect of quercetin by improving post-thawed sperm quality and fertility of rooster sperm.