Identification of RAPD primers specific for wheat homoeologous group 1 and 3 chromosomes.

Mapping of homoeologous chromosomes of common wheat (Triticum aestivum L.) has always been a top priority for wheat breeders. During present work, easier, cheaper and q uicker RA PD (Randomly Amplified Polymorphic DNA) primers were mapped on homoeologous group 1 and 3 chromosomes of common wheat. Wheat genetic stocks viz; Nullisomic-tetrasomic lines were used for mapping of RAPD primers. A total of 43 RAPD primers were tested, out of which, five primers viz; GLA-10, GLA-11, GLA-14, GLA-20 and GLB-15 were found useful and these primers amplified DNA fragments specific for chromosomes 1D, 3D, 1B, 3B and 1D, respectively. These five RAPD primers can reliably be used as marker s for the wheat homoeologous chromosomes 1B, 3B, 1D and 3D.