Immunolabelling of freeze-fractured tissue: thin sections of rat aorta after labelling of elastic laminae.

: Rat aortae were fixed, reduced into fragments, fixed again according to the fracture-label method from Pinto da Silva et al. (1981) and labelled with anti-elastin antibodies. We thus demonstrate that this method, extensively used with lectins, is also usable with antibodies to investigate components of the extracellular matrix.