Expression and significance ofether à go-go 1 channels in human ovarian cancer

Objective To evaluate the expression of Eag1 potassium channel in human ovarian cancer,the effects of Eag1 on human ovarian cancer cell line SKOV3 and the relationship between Eag1 expression and clinicopathological characteristics of ovarian cancer patients. Methods The expression of Eag1 in human ovarian cancer cell line SKOV3 was detected by quantificational reverse transcription polymerase chain reaction( qRT-PCR). The effects of astemizole( a inhibitor of Eag1) on SKOV3 cells proliferation and cycle were measured by MTT and ow cytometry. Then the expressions of Eag1 at protein level were detected by Western blot and immunohistochemical staining in 36 cases of ovarian cancer. The relationship between the index and clinicopathological parameters was analyzed. Results Eag1 was overexpressed in SKOV3 cells. Cell proliferation and cycle can be significantly inhibited by astemizole.Eag1 was expressed higher in the ovarian cancer specimens than in normal ovaries tissues and was associated with clinical stage( P0.05). Conclusion The expression of Eag1 potassium channel was aberrant in human ovarian cancer tissues and Eag1 potassium channel may represent a potential target for ovarian cancer diagnosis and treatment.