Coronene derivative probe and preparation method thereof, and protein detection method based on coronene derivative probe and aptamer

The invention provides a coronene derivative probe and a preparation method thereof and a protein detection method based on the coronene derivative probe and aptamer. The objective of the invention is to overcome the problems of tedious operation, complex process and low sensitivity of a conventional protein detection method. The probe is 1,2,7,8-tetra-(4-trimethylamine butyloxycarbonyl)-coronene and has four charges and water solubility. The invention provides the preparation method for the coronene derivative probe. The invention further provides the protein detection method based on the coronene derivative probe and the aptamer. The protein detection method is marker-free, simple and fast and does not need covalent modification; moreover, a fluorescence enhanced signal is given out in detection, so compared with a fluorescence weakened signal, higher sensitivity is obtained.