Studies on cellulase production by an isolated strain of Trichoderma viride Sn-9106 in solid-state fermination of residue of Chinese herbs

The Trichoderma viride Sn-9106 with high cellulase actibity was employed to produce enzyme on residues of Chinese herbs as substrate in solid state fermentation.Residues of Chinese herbs and peptone were found to be the best combination of carbon and nitrogen source for the production of cellulase.The nutrient composition of medium was optimized using response surface methodology(RSM).A fractional factorial design was applied to elucidate the nutrient medium components that significantly affect cellulase production.The composition of nutrient fermentation medium optimized by response surface methodology was as follow(g/L): wheat bran 19.80,peptone 2.06 and KH2PO42.90.Compared to the original medium,the cellulase activity increased from 8.83 IU/ml to 12.60 IU/ml.