Construction and immuno-protection evaluation of trivalent-epitope DNA vaccine pVIVO2-SjFABP/Sj26. SjGAPDH.

In Beijing,there has emerged an external invasion plant,Solanum rostratum dating from the beginning of this century.This plant originated from the North America.It is poisonous all over the body.There exists a sort of neurotoxin,solanine in Solanum rostratum.On the other hand,solanine is also a valuable material that,for example,is effective for inhibiting tumors.In order to utilize waste materials by obtaining pharmacologically and economically valuable products such as solanine from Solanum rostratum as well as becoming familiar with its toxic degree and tackling with this external invasion plant.It was designed to assess the tumor suppressing effect of Solanum rostratum by detecting the inhibiting effect of the whole-plant extract on the membrane ATPase activity of mouse tumor cell H22,with the functional effects of solanine and traditional chemotherapeutic cyclophosphamide as controls.The result revealed,in the nature of Beijing,the growing Solanum rostratum arising from invading expressed a tumor cell membrane ATPase activity inhibiting effect around as high as that of 3.63 mg/kg solanine,and around as high as or slightly higher than that of potato budlet equivalent.Therefore,it is possibly opportune to utilize waste material during renovating the ecology destroyed by the invading Solanum rostratum through obtaining medical components like solanine.It would be an economically efficient reaction,in the meanwhile emancipating the productive forces of potato and other valuable Solanum plants.