Radiosensitization ofcelecoxib on human lung adenocarcinoma cell line A549 and inhibition of migration abilityin vitro

：Objective To investigatethe effects of radiosensitivity enhancement and inhibition of migration ability of humanlung adenocarcinoma cells by celecoxib,a selective cyclooxygenase (COX)-2inhibitor.Methods Human lung adenocarcinoma cells of the line A549 were cultured and theninoculated into six-well plates and randomly divided into 4 groups:control group,celecoxibgroup administered with celecoxib at the subtoxic doses 30 and 50 μmol/L,irradiatedgroup exposed to 0,1,2,4,6,or 8 Gy by linear accelerator,and combined treatment (celecoxib+ irradiation) group.The radiosensitizing effect of celecoxib was assessed by clonogeniccell survival test.The migration ability of the A549 cells was measured by scratch-woundtest and the content of metalloproteinase-2 (MMP-2) in culture supernatant was detectedwith ELISA.Results The sensitization enhancement ratio of the celexib group was increaseddosedependently.The values of D0 ,Dq,SF2 and D0.01 of the celecoxib + irradiation groupwere all significantly lower than those of the irradiated group.Scratch-wound test showedthat the no-scratch area of the celecoxib + irradiation group and celecoxib group were allsignificantly wider than those of the mere irradiation and control groups and there was adose-dependent manner,and the no-scratch area of the celecoxib + irradiation group waswlider than that of the celecoxib group.ELISA showed that the MMP-2 levels in thesupernatant of the celecoxib group and celecoxib + irradiation group were respectivelysignificantly lower than those of the control group and mere irradiated group (t =3.78,5.79、3.15,P ＜ 0.05),however,there was notsignificant difference between the mere irradiation and control groups (t = 2.73,2.38,P＞ 0.05).ConclusionsCelecoxib enhances concentration-dependently the radiosensitivity of human lung carcinomacell and inhibits the secretion of MMP-2 of the carcinoma cells,thus inhibiting theirmigration ability. Key words: Lungneoplasms;  Radiosensitivity;  Cyclo-oxygenase-2 (COX-2); Metalloproteinase-2 (MMP-2)