Identification of novel protein band (s) distinguishing hulled v.s hullless varieties in barley (Hordeum vulgare L.) and its characterization

Barley (Hordeum vulgare) is one of the cereal crops among top five major crops of the world which is widely used for food, feed stock, malting, brewing and distilling industry. It belongs to the Poaceae family. The barley is cultivated as a Rabi crop in India with sowing being under taken from October-December and harvesting from March-May. Our aim was to quantify the protein of the hulled and hullless barley varieties and identify the novel protein band in hulled barley varieties. The range of seed grain protein in both hulled and hullless barley varieties were found 49.63 mg/gm. to 105.63 mg/gm. Maximum protein was found in Dolma i.e., 105.63 mg/gm, which minimum protein was found in NDB 1173 i.e., 49.63 mg/gm. Variation in presence or absence and the relative mobility of some electrophoretic band was also observed among the hulled and hullless barley genotype. The highest variation in the electrophoretic profile of seed storage protein was observed around at 70 kDa and 20 to 40 kDa range of band. All the Twelve barley genotypes hulled and hullless also differed in intensity of bands as well as in number of protein bands. To raise antibody against identified protein, rabbit was selected for immunization. A subcutaneous method was used for making protein titer for production of antibody.