Supplementation of Rosemary Leaves (Rosmarinus officinalis) Powder Attenuates Oxidative Stress, Inflammation and Fibrosis in Carbon Tetrachloride (CCl4) Treated Rats
2016
Objective: Rosmarinus officinalis (Family: Lamiaceae) has been used as a food preservative
and flavoring agent. This plant also contains antioxidant activity and can be used as a possible
therapeutic alternative for various diseases. The purpose of this study was to explore the antifibrotic
effect of Rosmarinus officinalis in carbon tetrachloride (CCl 4 )-induced liver dysfunction in rats.
Methods: Female Long Evans rats (10-12 weeks old) were divided equally into four different groups
such as control, control+rosemary, CCl 4 and CCl 4 +rosemary (6 rats in each group). Over the course
of the development of liver dysfunction, several oxidative stress parameters, increased liver marker
enzymatic functions were determined in CCl 4 treated rats. Moreover, histological assessments were
also done in liver section for inflammatory cells infiltration and fibrosis using hematoxylene and
eaosin staining and Sirius read staining.
Results: CCl 4 administration significantly increased the Alanine Aminotransferase (ALT), Aspartate
Aminotransferase (AST), and Alkaline Phosphatase (ALP) activities in plasma which was decreased
by Rosmarinus officinalis leaves powder supplementation. Rosmarinus officinalis prevented the rise
of lipid peroxidation, nitric oxide and Advanced Oxidative Protein Product (AOPP) in plasma and
liver tissues in CCl 4 treated rats. Histological studies also showed massive necrosis, periportal inflammation,
iron deposition and fibrosis in liver of CCl 4 treated rats which were further ameliorated
by Rosmarinus officinalis powder supplementation.
Conclusion: In conclusion, this study revealed the antifibrotic activity of Rosmarinus officinalis
powder on experimental hepatic damage and also suggests a potential therapeutic use of Rosmarinus
officinalis as an alternative therapy for hepatic disorders.
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