Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines

Prostate cancer is the second most frequently diagnosed cancer. Due to its high incidence and mortality, early diagnosis, identification of highly aggressive forms of clinically silent and understanding of disease pathogenesis with typical metabolic differences in order to develop specifically targeted therapy are needed. There have been found several compounds connected with tumourigenesis in prostate cells including Bcl-2. Bcl-2 acts as an inhibitor of apoptosis. It has been suggested that overexpression of Bcl-2 in human cancer cells contributes to their resistance to chemotherapy- and radiotherapy-induced apoptosis and is connected with unfavourable prognosis. In the fact, the majority of human prostate tumours overexpress Bcl-2. Possible associations with other proteins connected with tumour processes as c-Fos, c-Jun, Ki-67, NF-KB and p53 can be assumed. Therefore, we aimed our attention on determining of expression of Bcl-2, c-Fos, c-Jun, Ki-67,NF-KB and p53 genes in two prostate cell lines as 22Rvl cell line, a model of aggressive partially androgen-sensitive prostate cancer and PNTlA cell line, a model of healthy cell line. Moreover, we were interested in the issue how exposure of these cell lines to zinc(Il) ions could influence expression of the above mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation andhigh expression of Bcl-2.