Using Physicochemical Methods for in vitro Studying Urinary Calculi

Results of the application of X-ray diffraction studies, IR spectroscopy, thermogravimetry, spectrophotometry to the†in vitro†investigation of the phase composition of urinary calculi and the use of X-ray spectral microanalysis for the determination of their elemental composition are presented. The methods of X-ray diffraction and thermogravimetry were optimized for the quantitative determination of crystallization water in oxalates, and Lawry method was optimized for the quantitative determination of protein in all the kinds of urinary calculi. An interconnection between the hardness of oxalates, urates and phosphates, protein content and the kinds of microstructure was revealed.