Nucleic acid detection method for separating luminous marker based on magnetic beads and nucleic acid hydrolysis
2014
The invention discloses a nucleic acid detection method for separating a luminous marker based on magnetic beads and nucleic acid hydrolysis. The method comprises the following steps: designing a functional probe for detecting a target nucleic acid fragment; modifying the functional probe on the surface of functional magnetic beads; capturing the target nucleic acid fragment and a luminous marker to the surface of the magnetic beads by virtue of hybridization; performing magnetic separation; cleaning, thereby obtaining the magnetic beads on which the luminous marker is obtained; adding a nucleic acid hydrolysis reagent, separating the luminous marker from the surface of the magnetic beads by virtue of nucleic acid hydrolysis, thereby obtaining a luminous marker solution which does not contain the magnetic beads; and performing luminous detection on the solution, thereby acquiring the target nucleic acid information. According to the method disclosed by the invention, the luminous signal loss caused by a shielding effect of the magnetic beads on the luminous signal is completely overcome, 0.1pM of nucleic acid fragments can be detected at least, and the detection sensitivity is high.
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