Effect of Phosphorus Concentration on the Growth of Cattail Callus Cells

Abstract This investigation examined the growth of Typha latifolia (cattail) callus cells grown in five (0, 11, 22, 33, 44 mg L−1) different phosphorus concentrations. The cells were grown for two successive subcultures on semi‐solid media, and subsequently in suspension culture with the same phosphorus levels. On semi‐solid media, the fresh weight of the cells varied by a maximum of 36% through both subcultures. The 33 mg L−1 phosphorus supplied in the original Gamborgs B5 media promoted the greatest fresh weight of the cells in suspension culture over all other concentrations tested. When grown in suspension culture with 0 and 11 mg L−1 phosphorus, the cells showed a 42 and 29% reduction in fresh weight, respectively. Incubating the cells with 22 and 44 mg L−1 phosphorus caused a 20 and 13% reduction in fresh weight, respectively. In addition, this study compared the phosphorus concentration of callus cells against literature‐reported values of whole cattail plants incubated at similar phosphorus concen...