A Rapid and Economical Method for Low Molecular Weight RNA Isolation from a Wide Variety of Plant Species

We have developed a time- and cost-effective method for isolating low molecular weight (LMW) RNA from plants. In our protocol, the isolation procedure can be completed within 3 h. Polyethylene glycol (PEG) and absolute ethanol are used to isolate LMW RNA, and the LMW RNA yields were >80 µg/g of fresh-weight tissues for several of the plant species tested.