[Polymorphisms of inhibin α gene exon 1 in buffalo (Bubalus bubalis), gayal (Bos frontalis) and yak (Bos grunniens)].
2013
To elucidate the genetic characteristics of the bovine Inhibin α
subunit (INHA) gene, the polymorphisms in exon 1 of INHA and its
bilateral sequences were assayed using PCR with direct sequencing in
buffalo, gayal and yak. A comparative analysis was conducted by pooled
the results in this study with the published data of INHA on some
mammals including some bovine species together. A synonymous
substitution c.73C>A was identified in exon 1 of INHA for buffalo,
which results in identical encoding product in river and swamp buffalo.
In gayal, two non-synonymous but same property substitutions in exon 1
of INHA, viz. c.62 C>T and c.187 G>A, were detected, which lead
to p. P21L, p. V63M changes in INHA, respectively. In yak, nucleotide
substitution c.62C>T, c.129A>G were found in exon 1 of INHA, the
former still causes p. P21L substitution and the latter is synonymous.
For the sequence of the 5'-flanking region of INHA examined, no SNPs
were found within the species, but a substitution, c. -6T>G, was
found. The nucleotide in this site in gayal, yak and cattle was c. -6G,
whereas in buffalo it was c. -6T. Meanwhile, a 6-bp deletion, namely c.
262+31_262+36delTCTGAC, was found in the intron of buffalo INHA gene.
For this deletion, wild types (+/+) account for main part in river
buffalo while mutant types (-/-) are predominant in swamp buffalo. This
deletion was not found in gayal, yak and cattle, though these all have
another deletion in the intron of INHA, c. 262+78_262+79delTG. The
results of sequence alignment showed that the substitutions c. 43A and
c. 67G in exon 1 of INHA are specific to buffalo, whereas the
substitutions c. 173A and c. 255G are exclusive to gayal, yak and
cattle, and c. 24C, c. 47G, c. 174T and c. 206T are specific to goat.
Furthermore, there are few differences among gayal, yak and cattle, but
there relatively great differences between buffalo, goat and other
bovine species regarding the sequences of INHA exon 1.
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