[Tyrosine kinases inhibitor A77 1726 inhibits upregulation of collagen generation in fibroblasts induced by IL-13].

AIM:To observe the inhibitory effects of tyrosine kinases inhibitor A77 1726 on collagen generation induced by IL-13 in fibroblasts.METHODS:The inhibition rate of fibroblast proliferation with different concentration of A77 1726 was observed by MTT method.The fibroblasts were divided into the experimental group(A77 1726 50 μmol/L and IL-13 100 μg/L)and the control group(IL-13 100 μg/L).After 24,48 and 72 hours,the inhibitory effects of A77 1726 on collagen secretion of fibroblasts investigated by hydroxyproline release assay.The inhibitory effects of A77 1726 on collagen type I α1 gene mRNA expression in fibroblasts were examinated by RT-PCR.The influence of A77 1726 on collagen type I synthesis in fibroblasts was analyzed by Western blot.RESULTS:The proliferation of fibroblasts was inhibited by A77 1726.Total collagen generation was down-regulated after 48 h and 72 h stimulation of A77 1726(P0.05).The expression level of collagen type I α1 gene mRNA was obviously lower in the experimental group than in the control group after 48 h and 72 h stimulation of A77 1726(P0.05).Collagen type I production of fibroblasts treated with A77 1726 for 48 and 72 hours was decreased obviously in the experimental group than in the control group(P0.05).CONCLUSION:Tyrosine kinases inhibitor A77 1726 has an inhibitory effect on collagen protein synthesis in fibroblasts induced by IL-13.