S. cerevisiae protein disaggregation and synthesis estimates

Protein disaggregation and new synthesis measured by media-shift SILAC. Cells are grown on a first set of stable-isotope-labeled amino acids, shifted to media containing a second set of labels, then heat shocked at 42C for 10mins and allowed to recover at 30C for 0, 20, 60, or 180 minutes. Upon collection, these cells are mixed with cells from an unshocked (30C) reference sample grown on a third label. Protein in 100,000g supernatant fraction of lysate is measured, intensities and normalized ratios reported.