[The effect of hypoxia preconditioning no binding activity of HIF-1 on the HRE with EPO in the hippocampus of mice].

Aim:To observe change of binding activity of HIF-1 with erythropoietin(EPO) hypoxia response element(HRE) in the hippocampus of mice preconditioned to hypoxia and explore relationship between the changes and the preconditioning.Methods:The hippocampus was removed from mice exposed to hypoxia for 0 run(control group),1 run(H1 group) and 4 runs(H4 group).Electrophoretic mobility shift assays(EMSA),chromatin immunoprecipitation(ChIP)and real time PCR were used to detect the change of activity of HIF-1 on HRE of EPO.Results:Both in vitro and in vivo binding tests showed that the HIF-1 DNA-binding activities were increased in group H1 and markedly increased in group H4.Conclusion: The increase of HIF-1 and HRE of EPO binding activities is thought be involved in hypoxic preconditioning.