Study on Antioxidant Activity of Different Polarity Parts in Antheraea Pernyi in vitro

Objective The paper focused on silkworm pupa and the male zooid of Antheraea pernyi to investigate the anti-oxidation effect of the antheraea pernyi.Methods 80% ethanol extraction from silkworm pupa and the male zooid of Antheraea pernyi was partitioned by petroleum ether,butanol to afford three fractions.Using Spectrophotometric method,determination the antioxidant capacity of the total extracts,Petroleum ether layer,butanol layer and water layer by detecting the removing ratio to O-2·,·OH,DPPH·and ABTS+. Results Silkworm pupa and the male zooid of Antheraea pernyi have strong anti-oxidation activity,the 50% elimination value of total extraction to oxygen free radical,hydroxyl free radical,DPPH free radical and ABTS free radical were 8. 82324,12. 09121,0. 12991,0. 22706 and 8. 09633,14. 55845,2. 30111,10. 00324 g /L,respectively. In Polarity division of the experiment,Water layer butanol layer Petroleum ether layer. Conclusion Silkworm pupa and the male zooid of Antheraea pernyi have good ability of scavenging free radicals and have a prospective future of applications.