ANTIBACTERIAL ACTIVITY OF Kumis Kucing LEAF EXTRACT (Orthosiphon Stamineus) AGAINST Propionibacterium acnes BACTERIA

Acne is a skin disease that often affects teenagers to adults and a skin disease that almost happens to everyone. The main bacteria that causes acne is Propionibacterium acnes . Previous research stated that the extract of the Kumis Kucing leaf contains secondary metabolites have antibacterial inhibition is flavonoids, alkaloids, saponins, steroids and terpenoids. This study to determine the antibacterial activity extract of Kumis Kucing leaves against Propionibacterium acnes bacteria through MIC and MBC testing. The methods used in this study is a true experimental method with post test only with control group design. Propionibacterium acnes bacteria as research subjects were given 5 treatment groups is extracts of Kumis Kucing ( Orthosiphon Stamineus ) leaves with concentrations of 50%, 75%, and 100%, as well as positive control clindamycin and negative control DMSO 10%. The method in this study uses the disc diffusion method and liquid dilution. Antibacterial testing on the disc diffusion method obtained extracts of Kumis Kucing ( Orthosiphon stamineus ) leaves is the inhibition zone of 7.46 mm while the dilution method contained MIC at a concentration of 50% (0,5 g/10 ml), while the MBC was not obtained. The results of the statistical test Kruskal Wallis 0,018 and Mann Whitney 0,025 showed a p value <0,05, indicating that there was a significant difference between all treatments and between two variations in the concentration of Kumis Kucing ( Orthosiphon stamineus ) leaf extract against Propionibacterium acnes . The Conclusion is extract of Kumis Kucing ( Orthosiphon stamineus ) leaves against Propionibacterium acnes has inhibitory at a concentration of 50% but has not bactericidal.