High Performance Liquid Chromatography and its role in identification of Mycobacteriae: An Overview

HPLC has over the past decade become the method of choice for the analysis of a wide variety of compounds. Its main advantage over Gas Chromatography (GC) is that the analytes do not have to be volatile, so macromolecules are suitable for HPLC analysis. HPLC is accomplished by the injection of a small amount of liquid sample into a moving stream of liquid – the mobile phase, which passes through a column packed with particles of the stationary phase .