Quality control of target area in Yangyin Tongnao Granules by HPLC and UV-VIS

Objective: Determining the contents of general flavone of target area in Yangyin Tongnao Granules with ultraviolet-visible spectrophotometer(UV-VIS).Establish high-performance liquid chromatography(HPLC) to simultaneously detect the contents of puerarin,calycosin and formononetin,which are included in general flavone of target area in Yangyin Tongnao Granules.Methods: Rutin as controls product,determine the contents of general flavone of target area in Yangyin Tongnao Granules by UV-VIS.A HPLC method was set up with the gradient elution mode,using Eclipse XDB-C18,mobile phase was acetonitrile-0.04% phosphoric acid water,flow rate was 1.00mL/min,temperature was 30℃ with the wavelength at 250 nm.Results: The results show,the contents of general flavone of target area in Yangyin Tongnao Granules is above 70 percent.For puerarin,the calibration curve was linear(r=0.9998) in the range of 0.530-296.300mg/L,the average recovery was 99.8%,RSD=0.4%.For calycosin,the calibration curve was linear(r=0.9994) in the range of 0.064-44.440mg/L,the average recovery was 99.70%,RSD=1.10%.For formononetin,the calibration curve was linear(r=0.9994) in the range of 0.064-43.110mg/L,the average recovery was 99.40%,RSD=1.50%.Conclusion: This method is easy to operate and determination result is accurate and reliable,can be used for quality control of Yangyin Tongnao Granules.