Novelty of synergistic and cytotoxicity activities of silver nanoparticles produced by Lactobacillus acidophilus

Silver nanoparticles (AgNPs) have been recognized for their unique physical and chemical properties, silver nanoparticles (AgNPs) have identified themselves as one of the most shows potential nanoparticles in biomedical and biological applications, including anticancer, drug delivery, and antimicrobial agents. The release of [Ag +], which is toxic to microorganisms, could be responsible for AgNPs’ bactericidal properties. Synthesis of silver nanoparticles (NPs) using lactic acid bacteria could be a good environmentally friendly substitute for physical and chemical methods. Lactobacillus acidophilus was identified by biochemical and molecular methods and rummage sale for the green mixture of AgNPs. The AgNPs produced within the L. acidophilus culture medium were characterized by color change after L. acidophilus development by 1 mM AgNO3 at 37 °C. The NPs configuration was established by UV–visible at 434 nm. Characterization by transmission electron microscopy (TEM) proved that the sphere-shaped poly-dispersed AgNPs were in the scope variety of 19–25 nm. Fourier-transform infrared spectroscopy showed high levels of AgNP-steadying proteins and other subordinate metabolites. X-ray deflection exposed a face-centered cubic deflection spectrum by a crystal-like countryside. The antimicrobial action of AgNPs was verified against Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Salmonella enterica), Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), and a fungus (Candida albicans). The objective of this study was to investigate the biosynthesis of silver NPs using Lactobacillus and it is antibacterial and cytotoxicity activities of synthesized nanoparticles. The E. coli and S. enterica were highly susceptible, which were (21 and 20 mm), whereas S. aureus and C. albicans were the least susceptible, which were (10 and 9 mm). The findings on the cytotoxicity of AgNPs against Caco, A549, and HepG2 cell lines of cancer showed that the concentrations giving half-maximal responses were 5, 15, and 30 mg/ml, respectively. This study presents a well-organized and rapid method of synthesizing stable AgNPs using L. acidophilus with high antimicrobial and anticancer activities.