Effect of IgG gene silencing by RNA interference on radiosensitivity of prostate cancer PC3 cells

Objective To investigate the effect of RNA interference of Ig G gene on the radiosensitivity of the human prostate cancer PC3 cell line. Methods PC3 cells were trasnfected via lipofectamine by the sh RNA vector FCGR1 Ash RNA targeting the Fc segment of Ig G, using NCsh RNA as the negative control. Q-PCR and Western blotting were used to analyze the expression of Ig G in the trasnfected cells. The cells were then exposed to60 Co γ ray at 0, 2, 4, 6, 8, 10 Gy, and the cell proliferation was evaluated by MTS and the cells apoptosis estimated by flow cytometry at 12, 24 and 48 h. Results MTS assay showed that 60Coγ ray significantly inhibited the proliferation of PC3 cells transfected with FCGR1 Ash RNA as compared with NCsh RNAtransfected and blank control cells(P0.05). Flow cytometry showed that the cell apoptosis rate was significantly higher in FCGR1 Ash RNA group than in NCsh RNA and blank control groups at 48 h after γ ray exposure(P0.05). At 12, 24 and 48 h after 6 Gy radiation, the cells in FCGR1 Ash RNA group showed a significantly lowered proliferation rate and an increased apoptosis rate(P0.05). Conclusion The sh RNA targeting Ig G gene can significantly enhance the sensitivity of PC3 cells to radiation. The combination of RNA interference targeting Ig G gene with radiotherapy may be more effective in the treatment of prostate cancer.