Highly effective osteogenic conversion of mesenchymal Stem Cells by Cell Rev™ Supreme Osteo

Background & Aim Differentiation potentials of multipotent mesenchymal stem cells (MSCs) are one of the two primary hallmarks of MSCs. Media recipes have been formulated to enhance growth properties but often at the expense of differentiation potentials. Two major factors that are responsible for blunting MSC differentiation potential include the expansion as well as the differentiation media. The former can significantly hurdle MSCs properties especially in long term expanded MSCs. We aim to solve this major setback of MSCs quality; here we focus on osteogenic differentiation as bone repair by MSCs cell therapy is at the forefront of clinic use. Methods, Results & Conclusion Osteogenic differentiation is accounted by two cellular milieu: 1) the media that MSCs have been cultivated with and 2) the differentiation media to convert the MSCs to osteoblasts. We examine these issues experimentally by: comparing conversion rate and quality of osteogenic differentiation of A) MSCs grown on FBS derived standard media (STD) and MSCs has grown on Cell Rev™ MSC ExHQ 2.0 media, and B) comparing effectiveness of osteogenic differentiation of the MSCs that were cultivated on either industry-standard osteogenic differentiation kit (STD) or Cell Rev™ Supreme Osteo. First, Cell Rev™ Supreme Osteo increased MSCs conversion rates from 10% to 70% even if the MSCs were cultured in STD media. More interestingly, Cell Rev™ Supreme Osteo was able to achieve a 100% differentiation when the MSCs were expanded from Cell Rev™ MSC ExHQ 2.0. The results show synergistic effects of retaining high-level differentiation potential from maintaining MSCs in Cell Rev™ MSC ExHQ 2.0, and that contribute to the most effective osteoblast conversion when cultivated with Cell Rev™ Supreme Osteo. These results illustrate that Cell Rev™ Supreme Osteo is highly effective in converting MSCs to osteoblasts regardless of poor expansion media. This solution of complete osteoblast conversion is important for the safety issue of bone repair in clinical practices because it eliminates the risks of MSCs turning into unwanted types of cells.