Effect of glioma-associated oncogene 1 on the proliferation of human pancreatic cancer PANC-1 cells

Objective:To investigate the effect of glioma-associated oncogene 1(GLI1) on the proliferation ability of human pancreatic cancer PANC-1 cells.Methods:The recombinant lentiviral vector of RNA interference(RNAi)-targeting GLI1 gene was constructed.The pLVTHM-GLI1-shRNA or pLVTHM-GFP lentivirus(as the control)was infected into PANC-1 cells.The expressions of GLI1,bcl-2,bcl-xl and proliferating cell nuclear antigen(PCNA) mRNAs and proteins were detected by real-time fluorescence quantitative-PCR and Western blotting,respectively,and the cell growth was measured by cell count kit-8(CCK8) assay.Results:The GLI1-shRNA-PANC-1 cells with stable low-expression of GLI1 were successfully established.Compared with GFP-PANC-1 and PANC-1 cells,the expression levels of GLI1 mRNA and protein in GLI1-shRNA-PANC-1 cells were down-regulated by(82.1±3.2)% and(76.7±2.2)%,respectively(P0.01),and the expression levels of bcl-2 mRNA and protein were down-regulated(49.7±5.4)% and by(43.5±9.4)%,respectively(P0.01).There were no obvious changes in expressions of bcl-xl or PCNA mRNA and protein among three groups.The proliferation inhibitory rate of GLI1-shRNA-PANC-1 cells was higher than those in GFP-PANC-1 and PANC-1 cells(P0.05).Conclusion:GLI1 RNAi can inhibit the proliferation ability of pancreatic cancer PANC-1 cells.This effect may be associated with the down-regulation of bcl-2 expression.