Research of in vitro culture and regenerated system in anther of Northwest Japonica rice

In order to establish a stable and efficient in vitro culture and regenerated system in anther of Northwest Japonica rice,22 cultivars were studied on influence of genotype,culture medium,hormone,carbon sources and concentration using N6 culture medium and A4 culture medium.The result showed that genotype was ranked at the top of the factors,'3279' was high culture ability.Using N6 culture medium and A4 culture medium,A4 culture medium was better than N6 culture medium.Different genotypes had different culture abilities for concentration of 2,4-D,the extent was 1-3 mg / L.Sucrose was better to induce callus using anther,and 60 g/L was the best for proportion of sucrose to induce and differentiate callus.Although maltose was better to induce callus,green plantlet differentiation frequency was low.