The Combined Effect of Amniotic Membrane and Moringa oleifera Leaves Derived Gel for Wound and Burn Healing in Rat Model

A burn is a devastating phenomenon, especially in the developing countries. A bulk amount of medical resources is required to fulfill the demand of burned patients. So, it is very important to find another alternative for the treatment of burned patients. Recently, human amniotic membrane (AM) and Moringa oleifera (MO) are being used separately to treat wounds and burn. In this study, we tried to use a mix of AM and MO formulated to a gel with sodium carboxymethylcellulose (CMC-Na), propylparaben, glycerin, distilled water, and triethanolamine and applied on the burned skin of Wistar rats. Second-degree burns were created using pieces of aluminum (2.5-cm diameter). Skin irritation study, physiological properties, the percentage of wound contraction, and epithelialization period were measured for all groups. AM+MO-treated group had maximum average body weight, food intake, and water intake. At day 24, AM+MO group showed a greater response (p < 0.00003667) than other groups. For complete epithelialization, AM+MO required only 19.6 days while AM, MO, and control group needed 23.2, 28.2, and 31.4 days, respectively. These results suggested that the combined composition of AM+MO may be used as a good therapeutic agent to treat burn and wound. Since burn injury is a major clinical concern throughout the world, we performed a study with 20 rats to treat burns with minimum cost and quick healing rate. Three types of burn healing gels (AM gel, MO gel, and AM+MO combined gel) were prepared. The second-degree burn was created on the dorsal part of each rat’s skin and gels were used for that portion twice a day while Povisep solution was used in the control group. The rats of combined group (AM+MO) showed the maximum healing rate (96 ± 1.96%), maximum epithelialization (19.6 days), progressively body weight gain, and increased food and water intake.