Improved stimulated echo in diffusion magnetic resonance imaging: introducing a π pulse for SNR enhancement

PURPOSE: Anomalous diffusion in biological tissues can be examined by diffusion MRI for various applications, including tumor diagnosis and measurement of brain fiber pathways. However, the measurement of anomalous diffusion requires high b-values for the diffusion gradient in MRI, and current MRI methods cannot provide a high SNR. This study aimed to improve on the standard stimulated echo (STE) to enhance the SNR in diffusion MRI with high b-values. METHODS: Because of hardware limitations and human safety considerations, prolonging the diffusion time (Δ) is 1 of the few methods available to realize high b-values. Here, we propose a new echo mechanism for diffusion MRI to enhance SNRs under long Δ. By introducing a π pulse at the midpoint between 2nd and 3rd π/2 pulses of STE, we refocus the magnetic moment vectors in the longitudinal plane before the third π/2 pulse is applied, which preserves the full echo signals. This sequence was compared with STE and spin echo (SE). Nine Δs were tested in a phantom. Multi b-values with 2 Δs were tested in a mouse liver, brain, and tumor. RESULTS: Compared with STE and SE, the proposed improved STE (ISTE) exhibited an improved SNR in the phantom experiment and improved performance in the in vivo experiments. CONCLUSION: By using the proposed echo mechanism in diffusion MRI, we enhanced the SNR of the images, which enables us to investigate diffusion behavior at higher b-values and further facilitates the development of quantitative diffusion MRI and radiomics.