Expression and significance of NALP3 in the hypoxic lung lnjury in rats

Objective: To discuss the expression and significance of NALP3 (NACHT-LRR-PYDcontaining protein3) inflammasome in SD rats of acute lung injury in simulated hypoxic environment. Methods: Forty SD rats were randomly divided into 4 groups: control and 1, 3, 7 d groups respectively. The rats kept in hypoxic chamber which simulated the environment of 5 000 m altitude for 1, 3, 7 days. After that, the rats were sacrificed and lung tissues were obtained. Meanwhile we examined the arterial blood-gas, wet/dry mass ratio of lung tissues, and the pathological and ultrastructural changes of lungs were also been detected. At last, the protein expression levels of NALP3, caspase-1and IL-1β were analyzed by immunohistochemical method, the serum level of IL-1βwas tested 收稿日期 (Date of reception):2014-10-21 通信作者 (Corresponding author):吴岳,Email: qhmcwuyue@163.com 基金项目(Foundation item):青海大学自然科学团队项目(2012-QYY-2)。This work was supported by natural science team of Qinghai University (2012-QYY-2), P. R. China. doi: 10.3978/j.issn.2095-6959.2015.04.022 View this article at: http://dx.doi.org/10.3978/j.issn.2095-6959.2015.04.022 临床与病理杂志, 2015, 35(4) http://www.lcbl.net 636 高 原 低 氧 环 境 会 不 同 程 度 对 人 体 各 组 织 和 器官造成影响,以心肺损伤较严重 [ 1 。急性肺损 伤(ac u te l u ng i n j u r y,A L I)是指由严重的感染、 创 伤 、 休 克 、 缺 氧 等 原 因 引 起 的 包 括 肺 泡 上 皮 细 胞 、 肺 毛 细 血 管 内 皮 和 肺 间 质 的 急 性 弥 漫 性 损伤,其严重阶段即急性呼吸窘迫综合征 (a c u t e re s p i r ato r y d i s t re s s s y n d ro m e,A R D S)。相关研 究 表明,NA LP3(NACHT-LR R-P YD containing protein3)炎性体信号通路在ALI发生、发展中起着 重要作用,但NA LP3信号通路在低氧诱导的肺损 伤中的作用至今未见报道。本研究通过复制大鼠 高原低氧肺损伤模型,探讨NA LP3炎性体在低氧 急性肺损伤中的作用和机制,为高原低氧肺损伤 的治疗途径提供新的参考依据。