Integrating 'top-down' and 'bottom-up' mass spectrometric approaches for proteomic analysis of Shewanella oneidensis
2002
Here we present a comprehensive method for proteome analysis that integrates both intact protein measurement (“top-down”) and proteolytic fragment characterization (“bottom-up”) mass spectrometric approaches, capitalizing on the unique capabilities of each method. This integrated approach was applied in a preliminary proteomic analysis of Shewanella oneidensis, a metal-reducing microbe of potential importance to the field of bioremediation. Cellular lysates were examined directly by the “bottom-up” approach as well as fractionated via anion-exchange liquid chromatography for integrated studies. A portion of each fraction was proteolytically digested, with the resulting peptides characterized by on-line liquid chromatography/tandem mass spectrometry. The remaining portion of each fraction containing the intact proteins was examined by high-resolution Fourier transform mass spectrometry. This “top-down” technique provided direct measurement of the molecular masses for the intact proteins and thereby enabled...
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