Inhibitory Effects of NTF2 on Retinal Neovascularization in vitro

Objective To study the inhibitory effects of nuclear transport factor 2(NTF2) on retinal neovascularization in vitro.MethodsThe in vitro cultured human retinal capillary endothelial cells were stimulated with VEGF,and transfected recombinant adenovirus-associated NTF2(rAAV2-NTF2)(experimental group) or blank rAAV2(control group) in vitro.Flow cytometry and Boyden chamber assay were used to detect the effects of NTF2 on the VEGF-stimulated human retinal vascular endothelial cells proliferation and migration,respectively.ResultsFlow cytometry revealed that the cells in G1 phase in experimental and control groups accounted for(49.57±0.43)% and(27.21±0.86)%,respectively,with the difference being significant between them(P0.05).Boyden chamber assay demonstrated that the migrating number of cells in experimental and control groups was(13.8±1.5) and(34.6±2.5) respectively(n=6,P0.05),indicating NTF2 could inhibit the proliferation and migration of human human retinal capillary endothelial cells.ConclusionNTF2 can inhibit retinal neovascularization.