The Eukaryotic Expression of gK Gene of Pseudorabies Virus Ea Strain

The fragment containing gK gene coding domains was amplified by polymerase chain reaction(PCR) using BamH I 5′ fragment of PRV Ea strain as template.The fragment was cloned into pBluescriptIISK+,resulting recombination plasmid pSKgK.Then the gK gene was cloned into eukaryotic expression vector pEGFP-C1,following the EGFP(Enhanced Green Fluorescent Protein).The recombinant plasmid pEGFPgK were transfected into BHK-21 cell with lipofectin and selected under G418,until the normal BHK-21 cell die out.Expression protein was detected under inverted fluorescence microscope.The cells transfected pEGFPgk excited strong fluorescence in cytomembrane and cytoplasm,The results showed that gK gene was expressed in the cytomembrane and cytoplasm of BHK-21 cell.