Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea

Activity-based protein profiling (ABPP) is a chemical proteomics method to profile activity states of enzymes under physiological conditions. Here the authors show that ABPP can be applied to archaeal serine hydrolases in the model organism Sulfolobus acidocaldarius and can be used to identify novel putative serine hydrolases.