Simultaneous Determination of Index Components in Wuwei Shaji Oral Liquid by HPLC

Objective: To establish an HPLC method of detecting geniposide,mono-ammonium glycyrrhizinate,liquiritin,costunolide and dehydrocostus lactone in the Wuwei Shaji oral liquid simutaneously. Methods: Diamonsil C18( 2) column( 250 mm × 4. 6 mm,5μm) was used to analyze above components with mobile phase of acetonitrile( A)-0. 2 phosphoric acid( B) in gradient elution mode at the flow rate of 1. 0 m L / min under wavelength of 220 nm. Results: There was a good linear relationship of the five components,the RSD of the accuracy,repeatability,stability were all bellow 3% and the average recovery were 99. 95% 、95. 00% 、100. 26% 、99. 82% 、101. 33% 。Conclusion: This method is convenience,accuracy and specific,which can be a further supplement for the quality control of Wuwei Shaji oral liquid.