Analysis of amantadine in Laminaria Japonica and seawater of Daqin Island by ultra high performance liquid chromatography with positive electrospray ionization tandem mass spectrometry

Abstract A sensitive and validated method for determination of amantadine in Laminaria Japonica and seawater was established using ultra high performance liquid chromatography with positive electrospray ionization tandem spectrometry (UHPLC-ESI-MS/MS). Laminaria Japonica was extracted with acetonitrile containing formic acid (1%), then purified with 10.0 g anhydrous sodium sulfate, 0.50 g C18 and 0.50 g PSA powder. Seawater added 10.0 mL 0.20 mol/L hydrochloric acid was purified with MCX solid phase extraction (SPE) cartridge. After extraction and purification, the supernatant of Laminaria Japonica and eluate of seawater were evaporated to nearly dry under a gentle stream of nitrogen at 40 °C. Acetonitrile-0.1% formic acid in water (3/7, v/v) was adjusted to 1.00 mL final volume. An aliquot (10 μL) was injected into the C 18 column for separation with the mobile phase of acetonitrile and 0.1% formic acid in water at 0.25 mL·min −1 . Calibration curves were linear ranged from 1.00 ng/mL to 20.0 ng/mL. Mean recoveries were 73.5% to 95.8%, and limit of detection (LOD) and quantification (LOQ) were 0.50 μg/kg and 1.00 μg/kg for Laminaria Japonica . Mean recoveries were 75.8% to 93.4%, and LOD and LOQ were 0.50 ng/L and 1.00 ng/L for seawater. Based on the method above, Laminaria Japonica and seawater in Daqin Island were analyzed in February to June continuously, lgBAF3.71 (bioaccumulation factor), indicating a bioenrichment effect.