extemporaneous care product base of a microorganism of lyophilisate and surfactant (s) of HLB greater than or equal to 12

The present invention relates to a method of cosmetic treatment of a keratinous material comprising at least the steps of: (a) providing a lyophilisate containing at least one physiologically acceptable microorganism, live or inactivated, a metabolite thereof or one of its fractions and at least one surfactant of HLB greater than or equal to 12, (b) disposing a physiologically acceptable medium, distinct from said lyophilisate (c) performing the contacting of said lyophilizate extemporaneously with said medium under conditions conducive to the solubilizing and / or dispersing said freeze-dried product in said medium, and (d) contacting the mixture obtained in the previous step with said keratinique.Elle material further relates to a lyophilizate as defined above.