Determination of Vitamin D3 in the Fortified Sunflower Oil: Comparison of Two Developed Methods

Herein, the current investigation presents the study of two novel methods namely saponification liquid–liquid extraction coupled TLC (Sp-LLE-TLC) and direct liquid–liquid microextraction (LLME). These two methods were developed for enhanced determination of vitamin D3 in the fortified sunflower oil prior HPLC–UV analysis. By employing the proposed methods, linear dynamic range of 0.1–10 µg m/L with high determination coefficient of R2 > 0.94 was achieved in this work. Moreover, the obtained limit of detection and recovery values were found to be in reasonable range of 0.03–0.1 µg m/L and 71.7–101.44% (RSDs, 1.2–6.1%), respectively. Finally, by comparing two methods, the LLME was found to be green (organic solvent 500 µL), fast, and economic method for determination of vitamin D3 in the fortified sunflower oil as compared to Sp-LLE-TLC. Thus, the current work proposed a facile, economic, and green method for fast estimation of vitamin D3 in the fortified sunflower oil.