Screening of Camptothecin Production and SRAP Analysis of Endophytic Fungi from Camptotheca acuminata Decne

Endophytic fungi,lived in stems and leaves of host plants,were supposed to be effective or novel sources for therapeutic compounds.Camptotheca acuminata Decne is a traditional medical plant in China.About 50 endophytic fungi were isolated from barks of C.acuminata.A new fungus producing camptothecin(CPT) was identified as a Penicillium based on its morphological features.Ten endophytic fungi of C.acuminata were chosen to be amplified using molecular maker sequence related amplified polymorphism(SRAP).The combinations of SRAP primers turned out to be polymorphic and a total of 1 295 polymorphic bands were obtained.These strains were grouped into 3 main clusters and revealed that SRAP could isolate the strains of C.acuminata efficiently.It also provided evidence for discussing the genetic diversities of species in C.acuminata with SRAP molecular method.