Effect of simian vacuolating virus 40 on development and differentiation of dendritic cells from rhesus macaque.

To study the effect of simian vacuolating virus 40(SV40) on development and differentiation of dendritic cells(DC) from rhesus macaque,the peripheral blood-derived dendritic cells from rhesus monkey were pulsed with inactivated SV40 and infective SV40,respectively at the 5th day post DC cultivation.Expressions of CD1a,HLA-DR,CD86 and CD83 on the cell surface at the 7th,9th day post DC cultivation were analyzed by flow cytometry(FCM).The results showed that expressions of CD1a,HLA-DR,CD86 and CD83 on the cell surface in the inactivated SV40-pulsed experimental group were higher than those in the infective SV40-pulsed experimental group(P0.05).These cell surface molecules represented characteristic development and differentiation phase of DC.Down-regulation of expressions of these cell surface molecules indicated that infective SV40 might hamper differentiation and maturation of dendritic cells from rhesus monkey.