In silico detection of DNA methylation in fungi Neurospora crassa genes, rid-1 and dim-2

Neurospora crassa is a typical organism for many genetics study, DNA methylation is one of the most important process in DNA sequences which control the gene expression without changing DNA sequences. The Neurospora have a two genes dim-2 which is required for all known DNA methylation and rid-1 which is defeat any change in sequences. In silico program was used to analysis Neurospora DNA by designed two types of PCR primer, first set is for detection each gene and second set for detection CpG island in two types methylated and unmethylated primer. As well as using selective sensitive methylation restriction enzyme by draw an enzyme map by using Snapgene program tool.