Synergy of hematoporphyrin monomethyl ether and SonoVue on the killing of MDA-MB-231 cells under ultrasound irradiation: An experimental study

Objective To explore the synergy of hematoporphyrin monomethyl ether(HMME) and SonoVue on the killing of human breast cancer cell line MDA-MB-231 cells under ultrasound irradiation.Methods MDA-MB-231 cells in logarithmic growth phase were randomly divided into 8 groups(M group,H group,H+M group,U group,U+M group,U+H group,U+H+M group and control group).Under optimizing experimental conditions,MDA-MB-231 cells were irradiated with 50 Hz pulsed ultrasound for 60 s.Then the survival rate and morphological changes of MDA-MB-231 cells were detected respectively by MTT assay and inverted microscope.Result The survival rate of MDA-MB-231 cells of M group,H group,H+M group,U group,U+M group,U+H group,U+H+M group and control group was(91.90±1.41)%,(95.28±3.30)%,(90.76±4.01)%,(77.59±1.52)%,(52.12±2.90)%,(46.72±1.35)%,(31.47±1.48)%,and(99.95±0.66)%,respectively.Statistically significant differences were found between U+H+M group and any other group(all P0.01).Conclusion SonoVue can cooperate with HMME on the killing of MDA-MB-231 cells under ultrasound irradiation.