Cytotoxicity of three kinds of temporary crown and bridge materials on mouse fibroblasts in vitro

PURPOSE:This study was to investigate the cytotoxicity in vitro of three kinds of common temporary crown and bridge materials,i.e.,self-curing resin,heat-curing resin,and DMG-TEMP bi-acrylic composite.METHODS:L-929 mouse fibroblasts were used to evaluate the cytotoxicity of three kinds of temporary crown and bridge materials by agar overlay assay to test the cell death rate through trypan blue dyeing.RESULTS:The agar overlay assay showed that the value of Zone/Lysis of self-curing resin was 2/3,heat-curing resin was 1/0,and DMG-TEMP bi-acrylic composite was 1/2.Self-curing resin caused the most significant cytotoxicity(P0.05),followed by heat-curing resin and DMG-TEMP composite,and the latter two showed no significant difference(P0.05).The results of trypan blue dyeing were in accordance with the above.CONCLUSIONS:Self-curing resin,heat-curing resin,and DMG-TEMP bi-acrylic composite could decrease L-929 fibroblasts proliferation.Of all,self-curing resin caused most marked cytotoxicity,so we should avoid using self-curing resin as temporary crown and bridge to protect pulp.