In Vivo Photoacoustic Imaging of Nude Mice Vasculature Using a Photoacoustic Imaging System Based on a Commercial Ultrasound Scanner
2008
In-vivo photoacoustic/ultrasound (PA/US) imaging of nude mice was investigated using a photoacoustic imaging system
based on a commercial ultrasound scanner HDI-5000. Raw per-channel data was captured and beamformed to generate
each individual photoacoustic image with a single laser shot. An ultra-broadband CL15-7 linear array with a center
frequency of 8 MHz, combined with a Schott Glass fiber bundle, was used as a compact high resolution imaging probe,
with lateral and axial PA resolutions of about 300µm and 200µm, respectively. The imaging system worked in a dual
PA-US mode, with the ultrasound outlining the tissue structure and the photoacoustic image showing the blood vessels.
PA signals were generated by exposing mice to ultra-short optical pulses from a Nd:YAG-pumped OPO laser operating
in a wavelength range of 700-950nm. The corresponding ultrasound images were generated in the regular B-mode with
standard delay-and-sum beamforming algorithm. The system resolution was sufficiently high to identify and clearly
distinguish the dorsal artery and the two lateral veins in the mouse tail. Both the saphena artery and the ischiatic vein on
the cross-section of the mouse leg were clearly outlined in the PA images and correctly overlaid on the ultrasound image
of the tissue structure. Similarly, cross-section PA images of the mouse abdomen revealed mesenteric vasculatures
located below the abdominal wall. Finally, a successful PA imaging of the mouse thoracic cavity unveiled the ascending
and descending aorta. These initial results demonstrate a great potential for a dual photoacoustic/ultrasound imaging
modality implemented on a commercial ultrasound imaging scanner.
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