Morphological and Molecular Identification of a High Oleaginous Filamentous Fungi by rDNA-ITS2 Sequence

A filamentous fungal strain 7-4,which was screened out from 45 strains,was capable of producing high-yield lipids up to 46.8%,and identified by rDNA-ITS2 sequence and morphological observation.The sequence data was compared with the published sequences of relative filamentous fungal strains in Genbank.The results indicated that the homology of the rDNA-ITS2 sequences of strain 7-4 with those of several Mucor strains was more than 98.0%.As shown in the phylogenetic tree,strain7-4 and M.fragilis constituted a branch and they constituted a branch together with M.circinelloides.Furthermore,the strain 7-4 was similar to M.circinelloides in morphological character.Thus,the strain 7-4 was preliminarily determined to be belonged to the species of M.fragilis.The results showed that its composition of lipids was similar to vegetable oil and its unsaturated fatty acid content was 91.77%.This research therefore suggested that these microbes could be provided as raw material resource for microbial oil production.