Phyto-Analytical Evaluation of Rutin in Viola odorata L., (Banafshan)-An Expectorant

Viola odorata L., commonly known as banafshan, is widely distributed in Pakistan. Medicinally it used as expectorant. In present study a rapid and inexpensive qualification method for the quality control of Viola odorata on thin layer chromatography (TLC) was developed and validated on silica gel using solvent ethyl acetate–glacial acetic acid–formic acid-water (10 : 1.1 : 1.1 : 2.6 v/v)/v/v) (Rf of silybin 0.48 ± 0.05) in the absorbance mode at 254 nm. The method showed a good linear relationship (r2 = 0.999) in the concentration range 25–1500 ng per spot. It was found to be linear, accurate, precise, specifi c, robust and stability-indicating and can be applied for quality control and standardization. Also occurrence of rutin was figured out in different parts of plant. It was found that corolla of Viola odorata flower contain rutin in much higher concentration than calyx, while calyx have more rutin content than dried leaves, which in turn contain higher content of analyte than fresh leaves (figure- 1&2).